Customer Service

 Customer Survey

LABConnect™ - Online Results & More

Cryptococcus neoformans

Cryptococcus neoformans 

Cryptococcus neoformans (teleomorph: Filobasidiella spp.) is typically recovered from environmental samples as a nondescript yeast. This yeast is endemic throughout the world with different strains predominating in different locales. This yeast thrives in high nitrogen content material and is often associated with bird droppings. Outbreaks are typically associated with some kind of roosting site disturbance.

Cryptococcosis (an infection with Cryptococcus neoformans) is a trademark secondary infection in AIDS patients and most other cases are in patients with some other predisposing condition (such as lupus erythematosus, lymphoma, or leukemia). The infection is started by the inhalation of airborne fungal material and with ensuing infection in the lungs. The disease usually spreads to the brain and meninges, often proving to be fatal.

The main problem with the detection of Cryptococcus neoformans is that it is a nondescript yeast upon primary isolation. While most common indoor fungi can be differentiated to a degree by physical characteristics, yeasts are typically lumped into a single group. The differentiation of C. neoformans from other yeasts is based on biochemistry rather than direct observation and as such requires viable cultures of the organism. The analysis for C. neoformans can be performed in the typical 6-10 day turn around for viable cultures, but the test requires special media that do not allow other fungi to be reliably recovered or identified. Acceptable matrices include both swabs and bulk material are acceptable, as well as Andersen-style plates. For Andersen sampling the IAQ investigator should use Birdseed Agar (BBL) for the isolation medium, as it provides the quickest and most selective recovery of possible C. neoformans.

Culture Based Analysis:
EMSL Code: M028
TATs available: 2 weeks
Sample Types Accepted: Air (agar plates), swabs, and bulks.
Sample Types Not Accepted: Spore Traps, Tape Lifts, Soils
For Air Sample Collection: This method commonly uses an Andersen N-6 type impactor (e.g. EMSL VP-400 Microbial Sampler Product ID 8709001) with caffeic acid agar (CAA). 3-5 minute sampling at 28.3 lpm.
For Swab Sample Collection: Butterfield’s solution swab or similar
Sample Shipment: Complete an EMSL Chain of Custody (COC), available on our website (, detailing client name and information, project name or number, sample #, and a description of the area. Place samples in a cooler with reusable freezer packs. Overnight shipping recommended.

PCR Based Analysis:
EMSL Code: M143
TATs available: 3 hr, 6 hr, 1 day, 2 day, 3 day
Sample Types Accepted: Air (PCR cassette) and swabs
Sample Types Not Accepted: Spore Traps, Tape Lifts, Bulks, or Soils
For Air Sample Collection:  3-piece EMSL PCR cassette (Product ID 8715309) with vacuum pump. Sample as much air as desired through the upper opening. There is no upper limit to sampling time. Record the VOLUME of air sampled and ship the cassette to EMSL.
For Swab Sample Collection: Butterfield’s solution swab
Sample Shipment: Complete an EMSL Chain of Custody (COC), available on our website (, detailing client name and information, project name or number, sample #, and a description of the area. No refrigeration is needed. Overnight shipping recommended.


Full list of services provided for Cryptococcus neoformans ( click for details )
Cryptococcus neoformans
Identification of Viable Cryptococcus neoformans
Laboratories providing Cryptococcus neoformans ( click for details )
Atlanta, GA (LAB 07) - NVLAP Lab Code 101048-1Baton Rouge, LA (LAB 25) - NVLAP Lab Code 200375-0Beltsville, MD (LAB 19) - NVLAP Lab Code 200293-0Boston, MA (LAB 13) - NVLAP Lab Code 101147-0Buffalo, NY (LAB 14) - NVLAP Lab Code 200056-0Carle Place, NY (LAB 06) - NVLAP Lab Code 101048-10Charlotte, NC (LAB 41) - NVLAP Lab Code 200841-0Chicago, IL (LAB 26) - NVLAP Lab Code 200399-0Cinnaminson, NJ (LAB List in Description) - NVLAP Lab Code 101048-0Dallas, TX (LAB 11) - NVLAP Lab Code 600111-0Denver, CO (LAB 22) - NVLAP Lab Code 200828-0EMSL Canada - Calgary, AB (LAB 65) - NVLAP Lab Code 500100-0EMSL Canada - Edmonton, AB (LAB 50) - NVLAP Lab Code 600321-0EMSL Canada - Markham, ON (LAB 66) - NVLAP Lab Code 600317-0EMSL Canada - Montreal, QC (LAB 68) - NVLAP Lab Code 201052-0EMSL Canada - Ottawa, ON (LAB 67) - NVLAP Lab Code 201040-0EMSL Canada - Toronto, ON (LAB 55) - NVLAP Lab Code 200877-0EMSL Canada - Vancouver, BC (LAB 69) - NVLAP Lab Code 201068-0Fort Lauderdale, FL (LAB 56) - NVLAP Lab Code 500085-0Houston, TX (LAB 15) - NVLAP Lab Code 102106-0Huntington Beach, CA (LAB 33) - NVLAP Lab Code 101384-0Indianapolis, IN (LAB 16) - NVLAP Lab Code 200188-0Kernersville, NC (LAB 02) - NVLAP Lab Code 102104-0Las Vegas, NV (LAB 31) - NVLAP Lab Code 600140-0Long Island City, NY (LAB 03) - NVLAP Lab Code 101048-9Meriden, CT (LAB 24) - NVLAP Lab Code 200700-0Miami, FL (LAB 17) - NVLAP Lab Code 200204-0Minneapolis, MN (LAB 35) - NVLAP Lab Code 200019-0Ontario, California (San Bernadino County / Inland Empire) (LAB 71) - NVLAP Lab Code 600239-0Orlando, FL (LAB 34) - NVLAP Lab Code 101151-0Phoenix, AZ (LAB 12) - NVLAP Lab Code 200811-0Piscataway, NJ (LAB 05) - NVLAP Lab Code 101048-2Plymouth Meeting, PA (LAB 18) - NVLAP Lab Code 200699-0Raleigh, NC (LAB 29) - NVLAP Lab Code 200671-0Rochester, NY (LAB 53) - NVLAP Lab Code 600183-0San Diego, CA (LAB 43) - NVLAP Lab Code 200855-0San Leandro, CA (LAB 09) - NVLAP Lab Code 101048-3Santa Clara, CA (LAB 47) - NVLAP Lab Code 600318-0Seattle, WA (LAB 51) - NVLAP Lab Code 200613-0South Pasadena, CA (LAB 32) - NVLAP Lab Code 200232-0South Portland, ME (LAB 62) - NVLAP Lab Code 500094-0St. Louis, MO (LAB 39) - NVLAP Lab Code 200742-0Syracuse, NY (LAB 73) - NVLAP Lab Code 600283-0Tampa, FL (LAB 93) - NVLAP Lab Code 600215-0
Sorry, this function is disabled.